ORIGINAL PAPER
A comparison of the effect of chlorhexidine and collagen cross-linking
agent (oak extract) used for different times on composite-dentine
micro tensile bond strength
1
Department of Restorative Dentistry, School of Dentistry, Bushehr University of Medical Sciences, Bushehr, Iran
2
Department of Restorative Dentistry, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Submission date: 2021-02-01
Final revision date: 2021-03-14
Acceptance date: 2021-04-07
Publication date: 2021-06-30
Medical Studies 2021;37(2):125-130
KEYWORDS
TOPICS
ABSTRACT
Introduction:
he chief goal of restorative dentistry is to provide resin-dentin bond strength. Several materials have been proposed to strengthen the bond. External cross-link factors are a group of materials that improve the collagenic cross-links. Proanthocyanidins are a class of polyphenols found in a variety of plants including oak. Aim of the research: To compare the effect of chlorhexidine and collagen cross-linking agent (oak extract) used for different times on composite-dentine micro tensile.
Material and methods:
In the in vitro study, oak fruit was collected and used to make 33% oak extract. After that, 14 molar teeth were used, enamel was removed, and dentin was exposed. Then, 5 different protocols were used to prepare the teeth: 1. Using oak extract for 90 s, 2. Using oak extract for 180 s, 3. Using oak extract and chlorhexidine for 90 s, 4. Using oak extract and chlorhexidine for 180 s, and 5. Control group. Finally, the bonding strength was compared between the groups.
Results:
The maximum and minimum of bond strength were found in control and group 1 (24.86 vs. 19.49), respectively. All 4 intervention groups showed significant lower bonding strength than did the controls. The level of significance was lower than 0.0001. The least mean differences with controls (4.75) were seen in group 4.
Conclusions:
Our findings indicated that treatment with oak extract led to a decrease of the resin-dentin bond strength.
he chief goal of restorative dentistry is to provide resin-dentin bond strength. Several materials have been proposed to strengthen the bond. External cross-link factors are a group of materials that improve the collagenic cross-links. Proanthocyanidins are a class of polyphenols found in a variety of plants including oak. Aim of the research: To compare the effect of chlorhexidine and collagen cross-linking agent (oak extract) used for different times on composite-dentine micro tensile.
Material and methods:
In the in vitro study, oak fruit was collected and used to make 33% oak extract. After that, 14 molar teeth were used, enamel was removed, and dentin was exposed. Then, 5 different protocols were used to prepare the teeth: 1. Using oak extract for 90 s, 2. Using oak extract for 180 s, 3. Using oak extract and chlorhexidine for 90 s, 4. Using oak extract and chlorhexidine for 180 s, and 5. Control group. Finally, the bonding strength was compared between the groups.
Results:
The maximum and minimum of bond strength were found in control and group 1 (24.86 vs. 19.49), respectively. All 4 intervention groups showed significant lower bonding strength than did the controls. The level of significance was lower than 0.0001. The least mean differences with controls (4.75) were seen in group 4.
Conclusions:
Our findings indicated that treatment with oak extract led to a decrease of the resin-dentin bond strength.
REFERENCES (36)
1.
Mehran M, Faramarz Z, Nematolah FA, Fatemeh RS, Amir S. The comparison of effect of chlorhexidine and proanthocyanidin and grape seed extract on composite-dentin micro tensile bond strength: an in vitro experimental study. Jundishapur Sci Med J 2016; 15: 283-293.
2.
Yamauchi M. Collagen biochemistry: an overview. In: Advances in Tissue Banking Philips GO (ed). World Scientific 2002; 445-500.
3.
Monticelli F, Toledano M, Silva AS, Osorio E, Osorio R. Sealing effectiveness of etch-and-rinse vs self-etching adhesives after water aging: influence of acid etching and NaOCl dentin pretreatment. J Adh Dentistry 2008; 10: 183-188.
4.
De Munck J, Van Meerbeek B, Yoshida Y, Inoue S, Vargas M, Suzuki K, Lambrechts P, Vanherle G. Four-year water degradation of total-etch adhesives bonded to dentin. J Dental Res 2003; 82: 136-140.
5.
Hashimoto M, Fujita S, Endo K, Ohno H. In vitro degradation of resin–dentin bonds with one-bottle self-etching adhesives. Eur J Oral Sci 2009; 117: 611-617.
6.
Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin–dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials 2003; 24: 3795-3803.
7.
van Dijken JW, Sunnegårdh-Grönberg K, Lindberg A. Clinical long-term retention of etch-and-rinse and self-etch adhesive systems in non-carious cervical lesions: a 13 years evaluation. Dental Materials 2007; 23: 1101-1107.
8.
Tay FR, Pashley DH, Suh BI, Carvalho RM, Itthagarun A. Single-step adhesives are permeable membranes. J Dent 2002; 30: 371-382.
9.
Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, Ito S. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004; 83: 216-221.
10.
Donmez N, Belli S, Pashley DH, Tay FR. Ultrastructural correlates of in vivo/in vitro bond degradation in self-etch adhesives. J Dent Res 2005; 84: 355-359.
11.
Liu Y, Tjäderhane L, Breschi L, Mazzoni A, Li N, Mao J, Pashley DH, Tay FR. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J Dent Res 2011; 90: 953-968.
12.
Stanley A, Wilson M, Newman H. The in vitro effects of chlorhexidine on subgingival plaque bacteria. J Clin Periodontol 1989; 16: 259-264.
13.
Gendron R, Grenier D, Sorsa T, Mayrand D. Inhibition of the activities of matrix metalloproteinases 2, 8, and 9 by chlorhexidine. Clin Diagn Lab Immunol 1999; 6: 437-439.
14.
Bedran-Russo AKB, Pereira PNR, Duarte WR, Drummond JL, Yamauchi M. Application of crosslinkers to dentin collagen enhances the ultimate tensile strength. J Biomed Mater Res B Appl Biomater 2007; 80: 268-272.
15.
Han B, Jaurequi J, Tang BW, Nimni ME. Proanthocyanidin: a natural crosslinking reagent for stabilizing collagen matrices. J Biomed Mater Res A 2003; 65: 118-124.
16.
Ritter AV, Swift EJ, Yamauchi M. Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen. Eur J Oral Sci 2001; 109: 348-353.
17.
Buonocore MG. A simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J Dent Res 1955; 34: 849-853.
18.
Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res A 1982; 16: 265-273.
19.
Hashimoto M, Nagano F, Endo K, Ohno H. A review: biodegradation of resin–dentin bonds. Japan Dent Sci Rev 2011; 47: 5-12.
20.
Carrilho M, Carvalho RM, Tay FR, Yiu C, Pashley DH. Durability of resin-dentin bonds related to water and oil storage. Am J Dent 2005; 18: 315-319.
21.
Sheu MT, Huang JC, Yeh GC, Ho HO. Characterization of collagen gel solutions and collagen matrices for cell culture. Biomaterials 2001; 22: 1713-1719.
22.
Petite H, Rault I, Huc A, Menasche P, Herbage D. Use of the acyl azide method for cross-linking collagen-rich tissues such as pericardium. J Biomed Mater Res A 1990; 24: 179-187.
24.
Abdalla S, Pizzi A, Bahabri F, Ganash A. Analysis of Valonia oak (Quercus aegylops) acorn tannin and wood adhesives application. BioResources 2015; 10: 7165-7177.
25.
Nagpal R, Singh P, Singh S, Tyagi SP. Proanthocyanidin: a natural dentin biomodifier in adhesive dentistry. J Restor Dent 2016; 4: p. 1.
26.
Chang Y, Shin D. Effect of chlorhexidine application methods on microtensile bond strength to dentin in Class I cavities. Oper Dent 2010; 35: 618-623.
27.
Umachigi S, Jayaveera KN, Ashok Kumar CK, Kumar GS, Vrushabendraswamy BM, Kishore Kumar DV. Studies on wound healing properties of Quercus infectoria. Trop J Pharm Res 2008; 7: 913-9.
28.
Mapar M, Kalantari S. The comparison of effect of chlorhexidine and collagen cross linking agent (oak extract) on composite-dentine micro tensile bond strength. Dissertation. J AJUMS 2015; 1-30.
29.
Salminen JP, Roslin T, Karonen M, Sinkkonen J, Pihlaja K, Pulkkinen P. Seasonal variation in the content of hydrolyzable tannins, flavonoid glycosides, and proanthocyanidins in oak leaves. J Chem Ecol 2004; 30: 1693-1711.
30.
Castellan CS, Pereira PN, Grande RHM, Bedran-Russo AK. Mechanical characterization of proanthocyanidin–dentin matrix interaction. Dent Mater 2010; 26: 968-973.
31.
Leitune VCB, Portella FF, Bohn PV, Collares FM, Samuel SMW. Influence of chlorhexidine application on longitudinal adhesive bond strength in deciduous teeth. Braz Oral Res 2011; 25: 388-392.
32.
Loguercio AD, Stanislawczuk R, Polli LG, Costa JA, Michel MD, Reis A. Influence of chlorhexidine digluconate concentration and application time on resin–dentin bond strength durability. Eur J Oral Sci 2009; 117: 587-596.
33.
Popović BM, Stajner D, Zdero R, Orlović S, Galić Z. Antioxidant characterization of oak extracts combining spectrophotometric assays and chemometrics. Sci World J 2013; 2013: 134656.
34.
Liu Y, Chen M, Yao X, Xu C, Zhang Y, Wang Y. Enhancement in dentin collagen’s biological stability after proanthocyanidins treatment in clinically relevant time periods. Dent Mater 2013; 29: 485-492.
35.
Srinivasulu S, Vidhya S, Sujatha M, Mahalaxmi S. Shear bond strength of composite to deep dentin after treatment with two different collagen cross-linking agents at varying time intervals. Oper Dent 2012; 37: 485-491.
36.
Leme-Kraus A, Aydin B, Vidal CMP, Phansalkar RM, Nam JW, McAlpine J, Pauli GF, Chen S, Bedran-Russo AK. Biostability of the proanthocyanidins-dentin complex and adhesion studies. J Dent Res 2017; 96: 406-412.
| eISSN: | 2300-6722 |
| ISSN: | 1899-1874 |
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