ORIGINAL PAPER
Pharmaceutical availability of hydrogels with extracts of Arnica montana, Aesculus hippocastanum and Ruscus aculeatus and their potential use as antioxidant polyphenol-rich material
1
Department of Dermatology, Cosmetology and Aesthetic Surgery, Collegium Medicum, Jan Kochanowski University, Kielce, Poland
2
Department of Pharmacology, Collegium Medicum, Jan Kochanowski University, Kielce, Poland
3
Department of Organic and Physical Chemistry, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland
Submission date: 2023-03-09
Final revision date: 2023-08-08
Acceptance date: 2023-08-12
Publication date: 2023-09-30
Corresponding author
Monika Michalak
Dr Monika Michalak Department of Dermatology, Cosmetology and Aesthetic Surgery Collegium Medicum Jan Kochanowski University 19 a IX Wieków Kielc St, 25-317 Kielce, Poland
Dr Monika Michalak Department of Dermatology, Cosmetology and Aesthetic Surgery Collegium Medicum Jan Kochanowski University 19 a IX Wieków Kielc St, 25-317 Kielce, Poland
Medical Studies 2023;39(3):223-229
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Plant extracts are important sources of natural bioactive compounds, commonly used in the pharmaceutical and cosmeceutical industries. Phytochemical and antioxidant screening of plant extracts provides valuable information about their potential use. In the case of polyphenol-rich plant raw materials for external use, it is extremely important to test the ability of active substances to permeate the skin.
Aim of the research:
To determine the polyphenolic content and antioxidant activity of horse chestnut, butcher’s broom, and mountain arnica glycerol-water extracts and to assess the diffusion capacity of plant extracts from hydrogel formulations.
Material and methods:
The content of polyphenols was determined using a spectrophotometric method. Antioxidant properties were analysed using DPPH-EPR assay and the FRAP method. The pharmaceutical availability of the extracts in the hydrogels was tested using a simplified model of human skin represented by a cellulose dialysis membrane.
Results and conclusions:
The total polyphenol content in the extracts was highest in horse chestnut, followed by butcher’s broom and mountain arnica, whereas the arnica extract had the highest flavonoid content. The obtained results indicate that the extracts have high antioxidant potential, which can be ranked as follows: A. montana > A. hippocastanum > R. aculeatus. The pharmaceutical availability analysis showed that the extract from A. hippocastanum is released most efficiently. Analyses of the kinetics of active substance release from hydrogel formulations have proven to be a useful tool for assessing the efficacy of semi-solid products. Tested plant extracts can be used as polyphenol-rich natural materials with antioxidant properties.
Plant extracts are important sources of natural bioactive compounds, commonly used in the pharmaceutical and cosmeceutical industries. Phytochemical and antioxidant screening of plant extracts provides valuable information about their potential use. In the case of polyphenol-rich plant raw materials for external use, it is extremely important to test the ability of active substances to permeate the skin.
Aim of the research:
To determine the polyphenolic content and antioxidant activity of horse chestnut, butcher’s broom, and mountain arnica glycerol-water extracts and to assess the diffusion capacity of plant extracts from hydrogel formulations.
Material and methods:
The content of polyphenols was determined using a spectrophotometric method. Antioxidant properties were analysed using DPPH-EPR assay and the FRAP method. The pharmaceutical availability of the extracts in the hydrogels was tested using a simplified model of human skin represented by a cellulose dialysis membrane.
Results and conclusions:
The total polyphenol content in the extracts was highest in horse chestnut, followed by butcher’s broom and mountain arnica, whereas the arnica extract had the highest flavonoid content. The obtained results indicate that the extracts have high antioxidant potential, which can be ranked as follows: A. montana > A. hippocastanum > R. aculeatus. The pharmaceutical availability analysis showed that the extract from A. hippocastanum is released most efficiently. Analyses of the kinetics of active substance release from hydrogel formulations have proven to be a useful tool for assessing the efficacy of semi-solid products. Tested plant extracts can be used as polyphenol-rich natural materials with antioxidant properties.
REFERENCES (32)
1.
Michalak M. Plant-derived antioxidants: significance in skin health and the ageing process. Int J Mol Sci 2022; 23: 585-613.
2.
Dudek-Makuch M, Studzińska-Sroka E. Horse chestnut – efficacy and safety in chronic venous insufficiency: an overview. Rev Brasil Farmacogn 2015; 25: 533-541.
3.
Ionescu (Bordei) N, Neagu AM, Popescu M. Preparation and characterization of vegetable oils and plant extracts with effect in the treatment of varicose veins. UPB Sci Bull 2021; 83: 185-192.
4.
Kriplani P, Guarvea K, Baghael US. Arnica montana L. – a plant of healing: review. J Pharm Pharmacol 2017; 69: 925-945.
5.
Zheleva-Dimitrova D, Balabanova V. Antioxidant and acetylcholinesterase inhibitory potential of Arnica montana cultivated in Bulgaria. Turk J Biol 2012; 36: 732-737.
6.
Craciunescu O, Constantin D, Gaspar A, Toma L, Utoiu E, Moldovan L. Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts. Chem Cent J 2012; 6: 97-107.
7.
Petrova M, Zayova E, Vassilevska-Ivanova R, Vlahova M. Biotechnological approaches for cultivation and enhancement of secondary metabolites in Arnica montana L. Acta Physiol Plant 2012; 34: 1597-1606.
8.
Da Silva Prade J, Bálsamo EC, Machado FR, Rósula PM, Bortolotto VC, Araújo SM, Londero L, Boeira SP, Sehn CP, de Gomes MG, Prigol M, Souza LC. Anti-inflammatory effect of Arnica montana in a UVB radiation-induced skin-burn model in mice. Cutan Ocul Toxicol 2020; 39: 1‒23.
9.
Zampieron ER. Horse chestnut (Aesculus hippocastanum) for venous insufficiency. Int J Complement Alt Med 2017; 5: 14-12.
10.
Owczarek A, Kołodziejczyk-Czepas J, Marczuk P, Siwek J, Wąsowicz K, Olszewska MA. Bioactivity potential of Aesculus hippocastanum L. Flower: phytochemical profile, antiradical capacity and protective effects on human plasma components under oxidative/nitrative stress in vitro. Pharmaceuticals 2021; 14: 1301-1317.
11.
Aksoy H, Çevik Ö, Şen A, Göğer F, Şekerler T, Şener A. Effect of Horse-chestnut seed extract on matrix metalloproteinase-1 and -9 during diabetic wound healing. J Food Biochem 2019; 43: 1745-1514.
12.
Fujimura T, Tsukahara K, Moriwaki S, Hotta M, Kitahara T, Takema Y. A horse chestnut extract, which induces contraction forces in fibroblasts, is a potent anti-aging ingredient. J Cosmet Sci 2006; 57: 369-376.
13.
Masullo M, Pizza C, Piacente S. Ruscus Genus: A rich source of bioactive steroidal saponins. Planta Med 2016; 82: 1513-1524.
14.
Poljuha D, Šola I, Bilic J, Dudaš S, Bilušić T, Markić J, Rusak G. Phenolic composition, antioxidant capacity, energy content and gastrointestinal stability of Croatian wild edible plants. Eur Food Res Technol 2015; 241: 573-585.
15.
Rodrigues JPB, Fernandes Â, Dias MI, Pereira C, Pires TCSP, Calhelha RC, Carvalho AM, Ferreira ICFR, Barros L. Phenolic compounds and bioactive properties of Ruscus aculeatus L. (Asparagaceae): the pharmacological potential of an underexploited subshrub. Molecules 2021; 26: 1882-1894.
16.
Kowalska G, Baj T, Kowalski R, Szymańska J. Optimization of glycerol–water extraction of selected bioactive compounds from peppermint and common nettle. Antioxidants 2021; 10: 817-835.
17.
Agbor GA, Vinson JA, Donnelly PE. Folin-ciocalteau reagent for polyphenolic assay. Int J Food Sci Nutr Diet 2014; 3: 801-810.
18.
Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 2005; 91: 571-577.
19.
Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003; 81: 321-326.
20.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 1996; 239: 70-76.
21.
Szcześniak M, Pluta J. Infuence of hydrophiling agents on gel formation rate on Carbopol base. Polim Med 2011; 41: 27-32.
22.
Polish Pharmacopoeia edition X. Institute for Drug Research & Control, Poland Warsaw 2014.
23.
Hudemowicz W, Piotrowska I, Sieradzki E. Ointment. Transdermal therapeutic systems. Exercises with drug form technology. Medical University of Warsaw Warsaw, Poland 2014.
24.
Dudek-Makuch M, Studzińska-Sroka E, Korybalska K, Czepulis N, łuczak J, Rutkowski R, Marczak ł, Długaszewska J, Grabowska K, Stobiecki M, Cielecka-Piontek J, Bylka W, Witowski J. Biological activity of Aesculus hippocastanum flower extracts on vascular endothelial cells cultured in vitro. Phytochem Lett 2019; 30: 367-375.
25.
De Almeida PA, Alves MC, Polonini HC, Dutra LS, Leite MN, Raposo NRB, de O Ferreira A, Brandao MAF. New HPLC method for quality control of -escin in Aesculus hippocastanum L. Hydroalcoholic extract. Lat Am J Pharm 2013; 32: 1082-1087.
26.
Jakovljević VD, Milićević JM, Ðelić GT, Vrvić MM. Antioxidant activity of Ruscus species from Serbia: potential new sources of natural antioxidants. Hemijska Industrija Hem Ind 2016; 70: 99-106.
27.
Zillich OV, Schweiggert-Weisz U, Eisner P, Kerscher M. Polyphenols as active ingredients for cosmetic products. Int J Cosmet Sci 2015; 37: 455-464.
28.
Zillich OV, U Schweiggert-Weisz U, Hasenkopf K, Eisner P, Kerscher M. Release and in vitro skin permeation of polyphenols from cosmetic emulsions. Int J Cosmet Sci 2013; 35: 491-501.
29.
Piechota-Urbańska M, Berner-Strzelczyk A. Glycol plant extracts in the prescription of topical skin-whitening hydrogels. Polim Med 2011; 41: 17-25.
30.
Zagórska-Dziok M, Sobczak M. Hydrogel-based active substance release systems for cosmetology and dermatology application: a review. Pharmaceutics 2020; 12: 396‒439.
31.
Kasperek-Nowakiewicz R. Hydrożele w preparatyce farmaceutycznej. Aptekarz Polski 2019; 154: 7-11.
32.
Ali SM, Yosipovith G. Skin pH: from basic science to basic skin care. Acta Derm Venereol 2013; 93: 261-267.
| eISSN: | 2300-6722 |
| ISSN: | 1899-1874 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
