ORIGINAL PAPER
Therapeutic effect of Ziziphus lotus honey on hard palate ulcers
in rats
1
Department of Periodontology, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2
Department of Pathology, School of Dentistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3
Medicinal Plant Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Submission date: 2020-12-11
Final revision date: 2021-02-22
Acceptance date: 2021-03-04
Publication date: 2021-03-31
Medical Studies 2021;37(1):58-64
KEYWORDS
TOPICS
ABSTRACT
Introduction:
The aim of the study was to assess the effect of honey gel on the hard palate wound healing process in rats.
Material and methods:
In this interventional study, a total of 60 rats were randomly divided into 4 groups (n = 15) including a control group, a honey gel group, a polyethylene glycol base group, and an oral honey group. Oral wounds were created (4 × 6 mm) on the anterior palatal rugae, and after 24 h the wounds were rinsed with normal saline solution and the studied groups were treated by topical application of honey gel, polyethylene glycol base, and oral honey. On days 3, 7, and 14, five rats from each group were euthanized and histological samples were prepared, and the tissue slides were stained with haematoxylin eosin and observed under a light microscope. The mean number of neutrophils, fibroblasts, macrophage and blood vessel count, epithelialization, and the density of collagen fibres of each sample were assessed. Data were analysed using ANOVA and Kruskal-Wallis Test.
Results:
The number of blood vessels and the density of collagen fibres on day 14 were significantly higher in the honey gel group than in the control group (p < 0.05). The number of blood vessels and the rate of epithelialization on day 14 were significantly increased in honey gel group compared to basal group (p < 0.05). Other data were not statistically significant among the groups (p > 0.05).
Conclusions:
Topical application of honey gel can accelerate the wound healing process of the hard palate.
The aim of the study was to assess the effect of honey gel on the hard palate wound healing process in rats.
Material and methods:
In this interventional study, a total of 60 rats were randomly divided into 4 groups (n = 15) including a control group, a honey gel group, a polyethylene glycol base group, and an oral honey group. Oral wounds were created (4 × 6 mm) on the anterior palatal rugae, and after 24 h the wounds were rinsed with normal saline solution and the studied groups were treated by topical application of honey gel, polyethylene glycol base, and oral honey. On days 3, 7, and 14, five rats from each group were euthanized and histological samples were prepared, and the tissue slides were stained with haematoxylin eosin and observed under a light microscope. The mean number of neutrophils, fibroblasts, macrophage and blood vessel count, epithelialization, and the density of collagen fibres of each sample were assessed. Data were analysed using ANOVA and Kruskal-Wallis Test.
Results:
The number of blood vessels and the density of collagen fibres on day 14 were significantly higher in the honey gel group than in the control group (p < 0.05). The number of blood vessels and the rate of epithelialization on day 14 were significantly increased in honey gel group compared to basal group (p < 0.05). Other data were not statistically significant among the groups (p > 0.05).
Conclusions:
Topical application of honey gel can accelerate the wound healing process of the hard palate.
REFERENCES (38)
1.
Wyrębek B, Górski B, Górska R. Patient morbidity at the palatal donor site depending on gingival graft dimension. Dental Med Problems 2018; 55: 153-159.
2.
Nwman MG, Takei H, Klokkevold PR, Carranza FA. Newman and Carranza’s Clinical Periodontology E-Book: Elsevier Health Sciences 2018.
3.
Freedman M, Stassen LF. Commonly used topical oral wound dressing materials in dental and surgical practice – a literature review. Irish Med J 2013; 59: 190-195.
4.
Clark M, Adcock L. Honey for Wound Management: A Review of Clinical Effectiveness and Guidelines [Internet]. Ottawa (ON): Canadian Agency for Drugs and Technologies in Health; 2018 Nov 6. Available from: https://www.ncbi.nlm.nih.gov/b....
5.
Chamani G, Zarei MR, Mehrabani M, Mehdavinezhad A, Vahabian M, Ahmadi-Motamayel F. Evaluation of honey as a topical therapy for intraoral wound healing in rats. Wounds 2017; 29: 80.
6.
Aziz Z, Hassan BAR. The effects of honey compared to silver sulfadiazine for the treatment of burns: a systematic review of randomized controlled trials. Burns 2017; 43: 50-57.
7.
Wahdan H. Causes of the antimicrobial activity of honey. Infection 1998; 26: 26-31.
8.
Nazeri S, Ardakani EM, Babavalian H, Latifi AM. Evaluation of effectiveness of honey-based alginate hyrogel on wound healing in a mouse model of rat. J Appl Biotechnol Rep 2015; 2: 293-297.
9.
Ramsay EI, Rao S, Madathil L, Hegde SK, Baliga-Rao MP, George T, Baliga MS. Honey in oral health and care: a mini review. J Oral Biosci 2019; 61: 32-36.
10.
Beena JP, Sahoo P, Konde S, Raj NS, Kumar NC, Agarwal M. Manuka honey: a potent cariostatic agent – an in vitro study. Int J Clin Pediatr Dent 2018; 11: 105-109.
11.
Asadi-Pooya AA, Pnjehshahin MR, Beheshti S. The antimycobacterial effect of honey: an in vitro study. Riv Biol 2003; 96: 491-495.
12.
Nayak PA, Nayak UA, Mythili R. Effect of Manuka honey, chlorhexidine gluconate and xylitol on the clinical levels of dental plaque. Contemp Clin Dentistry 2010; 1: 214-217.
13.
Singhal R, Siddibhavi M, Sankeshwari R, Patil P, Jalihal S, Ankola A. Effectiveness of three mouthwashes–Manuka honey, Raw honey, and Chlorhexidine on plaque and gingival scores of 12–15-year-old school children: a randomized controlled field trial. J Indian Soc Periodontol 2018; 22: 34-39.
14.
Atwa ADA, AbuShahba RY, Mostafa M, Hashem MI. Effect of honey in preventing gingivitis and dental caries in patients undergoing orthodontic treatment. Saudi Dental J 2014; 26: 108-114.
15.
Drain J, Fleming MO. Palliative management of malodorous squamous cell carcinoma of the oral cavity with Manuka honey. J Wound Ostomy Continence Nurs 2015; 42: 190-192.
16.
Rao S, Hegde SK, Rao P, Dinkar C, Thilakchand KR, George T, Baliga-Rao MP, Palatty PL, Baliga MS. Honey mitigates radiation-induced oral mucositis in head and neck cancer patients without affecting the tumor response. Foods 2017; 6: 77.
17.
Charalambous M, Raftopoulos V, Paikousis L, Katodritis N, Lambrinou E, Vomvas D, Georgiou M, Charalambous A. The effect of the use of thyme honey in minimizing radiation-induced oral mucositis in head and neck cancer patients: a randomized controlled trial. Eur J Oncol Nurs 2018; 34: 89-97.
18.
Charalambous A, Lambrinou E, Katodritis N, Vomvas D, Raftopoulos V, Georgiou M, Paikousis L, Charalam-bous M. The effectiveness of thyme honey for the management of treatment-induced xerostomia in head and neck cancer patients: a feasibility randomized control trial. Eur J Oncol Nurs 2017; 27: 1-8.
19.
Ghashm AA, Othman NH, Khattak MN, Ismail NM, Saini R. Antiproliferative effect of Tualang honey on oral squamous cell carcinoma and osteosarcoma cell lines. BMC Complement Altern Med 2010; 10: 49.
20.
Bogdanov S. Propolis. Composition, Health, Medicine A Review. Bee Product Science 2017; 1-28.
21.
Al-Waili N, Salom K, Al-Ghamdi AA. Honey for wound healing, ulcers, and burns; data supporting its use in clinical practice. TheScientificWorldJournal 2011; 11: 766-787.
22.
Gill R, Poojar B, Bairy LK, Praveen KS. Comparative evaluation of wound healing potential of manuka and acacia honey in diabetic and nondiabetic rats. J Pharm Bioallied Sci 2019; 11: 116-126.
23.
Rao PV, Krishnan KT, Salleh N, Gan SH. Biological and therapeutic effects of honey produced by honey bees and stingless bees: a comparative review. Rev Brasil Farmacogn 2016; 26: 657-664.
24.
George Broughton I, Janis JE, Attinger CE. Wound healing: an overview. Plast Reconstr Surg 2006; 117 (7 Suppl): 1e-S-32e-S.
25.
Simpson DM, Ross R. The neutrophilic leukocyte in wound repair: a study with antineutrophil serum. J Clin Investig 1972; 51: 2009-2023.
26.
Oryan A, Zaker S. Effects of topical application of honey on cutaneous wound healing in rabbits. J Veter Med Series A 1998; 45: 181-188.
27.
Meszaros AJ, Reichner JS, Albina JE. Macrophage-induced neutrophil apoptosis. J Immunol 2000; 165: 435-441.
28.
Nakajima Y, Nakano Y, Fuwano S, Hayashi N, Hiratoko Y, Kinoshita A, Miyahara M, Mochizuki T, Nishino K, Tsuruhara Y, Yokokawa Y, Iuchi T, Kon Y, Mukai K, Kitayama Y, Murakado N, Okuwa M, Nakatani T. Effects of three types of Japanese honey on full-thickness wound in mice. Evid Based Complement Alternat Med 2013; 2013: 504537.
29.
Haryanto TU, Mukai K, Suriadi JS, Nakatani T. Effectiveness of Indonesian honey on the acceleration of cutaneous wound healing: an experimental study in mice. Wounds 2012; 24: 110-119.
30.
Bingle L, Brown N, Lewis C. The role of tumour-associated macrophages in tumour progression: implications for new anticancer therapies. J Pathol 2002; 196: 254-265.
31.
Fukuda M, Kobayashi K, Hirono Y, Miyagawa M, Ishida T, Ejiogu EC, Sawai M, Pinkerton KE, Takeuchi M. Jungle honey enhances immune function and antitumor activity. Evid Based Complement Altern Med 2011; 2011: 908743.
32.
Nisbet HO, Nisbet C, Yarim M, Guler A, Ozak A. Effects of three types of honey on cutaneous wound healing. Wounds 2010; 22: 275-283.
33.
Molan PC. The role of honey in the management of wounds. J Wound Care 1999; 8: 415-418.
34.
Suguna L, Chandrakasan G, Joseph KT. Influence of honey on collagen metabolism during wound healing in rats. J Clin Biochem Nutrition 1992; 13: 7-12.
36.
Gupta S, Singh H, Varshney A, Prakash P. Therapeutic efficacy of honey in infected wounds in buffaloes. Indian J Animal Sci 1992; 62: 521-523.
37.
Subrahmanyam M. A prospective randomised clinical and histological study of superficial burn wound healing with honey and silver sulfadiazine. Burns 1998; 24: 157-161.
38.
Mak K, Manji A, Gallant-Behm C, Wiebe C, Hart DA, Larjava H, Hakkinen L. Scarless healing of oral mucosa is characterized by faster resolution of inflammation and control of myofibroblast action compared to skin wounds in the red Duroc pig model. J Dermatol Sci 2009; 56: 168-180.
| eISSN: | 2300-6722 |
| ISSN: | 1899-1874 |
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