ORIGINAL PAPER
Potential cytotoxic effect of cannabidiol against human glioblastoma cells in vitro
1
Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland
These authors had equal contribution to this work
Submission date: 2024-02-23
Acceptance date: 2024-03-20
Publication date: 2024-09-13
Corresponding author
Jacek Kurzepa
Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland
Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland
Medical Studies 2024;40(4):336-340
KEYWORDS
TOPICS
ABSTRACT
Introduction::
In addition to its antiepileptic and anti-inflammatory properties, cannabidiol (CBD), a non-psychoactive component of cannabis, also has potential anticancer properties.
Aim of the research::
Evaluation the effect of CBD on the cell viability of 2 human glioblastoma lines.
Material and methods::
LN18 and LN229 cell lines relative to the physiological primary oligodendrocyte line MO3.13 were included in the study. The cells were exposed to CBD at increasing doses of 5, 10, 15, 20, 25, and 30 μM for 24 and 48 h. Cell viability after the planned incubation period was assessed using the commercial MTT test. Statistical calculations were evaluated with usage of Statistics Kingdom web application, and the IC50 value was assessed with usage of Quest Graph™ IC50 Calculator AAT Bioquest, Inc.
Results::
A dose-dependent cytotoxic activity of CBD was found in reducing the viability of each of the tested cell types. The cytotoxic effect was comparable after 24 and 48 h of incubation. The IC50 value was the lowest for LN229 line cells and amounted to 8.9 μM and 8.8 μM after 24 and 48 h of incubation, respectively, and the highest for the MO3.13 line and amounted to 12.4 μM and 13.5 μM after 24 and 48 h of incubation, respectively.
Conclusion::
CBD exerts a cytotoxic effect against glioblastoma cells. However, the reduction of viability also of physiological cell line suggests that the observed effect may not be selective for glioblastoma.
In addition to its antiepileptic and anti-inflammatory properties, cannabidiol (CBD), a non-psychoactive component of cannabis, also has potential anticancer properties.
Aim of the research::
Evaluation the effect of CBD on the cell viability of 2 human glioblastoma lines.
Material and methods::
LN18 and LN229 cell lines relative to the physiological primary oligodendrocyte line MO3.13 were included in the study. The cells were exposed to CBD at increasing doses of 5, 10, 15, 20, 25, and 30 μM for 24 and 48 h. Cell viability after the planned incubation period was assessed using the commercial MTT test. Statistical calculations were evaluated with usage of Statistics Kingdom web application, and the IC50 value was assessed with usage of Quest Graph™ IC50 Calculator AAT Bioquest, Inc.
Results::
A dose-dependent cytotoxic activity of CBD was found in reducing the viability of each of the tested cell types. The cytotoxic effect was comparable after 24 and 48 h of incubation. The IC50 value was the lowest for LN229 line cells and amounted to 8.9 μM and 8.8 μM after 24 and 48 h of incubation, respectively, and the highest for the MO3.13 line and amounted to 12.4 μM and 13.5 μM after 24 and 48 h of incubation, respectively.
Conclusion::
CBD exerts a cytotoxic effect against glioblastoma cells. However, the reduction of viability also of physiological cell line suggests that the observed effect may not be selective for glioblastoma.
REFERENCES (21)
1.
Radwan MM, Chandra S, Gul S, ElSohly MA. Cannabinoids, phenolics, terpenes and alkaloids of cannabis. Molecules. 2021; 26(9). 2774.
2.
Wheeldon J, Heidt J. Cannabis, research ethics, and a duty of care. Res Ethics. 2023; 19(3): 250-287.
3.
O’Sullivan SE, Jensen SS, Nikolajsen GN, Bruun HZ, Bhuller R, Hoeng J. The therapeutic potential of purified cannabidiol. J Cannabis Res. 2023; 5: 21.
4.
Corsato Alvarenga I, Wilson KM, McGrath S. Tolerability of long-term cannabidiol supplementation to healthy adult dogs. J Vet Intern Med. 2024; 38(1): 326-335.
5.
Calapai F, Esposito E, Ammendolia I, Mannucci C, Calapai G, Currò M, Cardia L, Chinou I. Pharmacovigilance of unlicensed cannabidiol in European countries. Phytother Res. 2023; 38(1): 74-81.
6.
Seltzer ES, Watters AK, MacKenzie Jr D, Granat LM, Zhang D. Cannabidiol (CBD) as a promising anti-cancer drug. Cancers (Basel). 2020; 12(1): 3203.
7.
Xiong Z, Raphael I, Olin M, Okada H, Li X, Kohanbash G. Glioblastoma vaccines: past, present, and opportunities. EBioMedicine. 2024; 100: 104963.
8.
Małek A, Kocot J, Mitrowska K, Posyniak A, Kurzepa J. Bee venom effect on glioblastoma cells viability and gelatinase secretion. Front Neurosci. 2022; 16: 792970.
9.
Khodadadi H, Salles ÉL, Alptekin A, Mehrabian D, Rutkowski M, Arbab AS, Yeudall WA, Yu JC, Morgan JC, Hess DC, Vaibhav K, Dhandapani KM, Baban B. Inhalant cannabidiol inhibits glioblastoma progression through regulation of tumor microenvironment. Cannabis Cannabinoid Res. 2021; 8(5): 824-834.
10.
Likar R, Koestenberger M, Stutschnig M, Nahler G. Cannabidiol μay prolong survival in patients with glioblastoma multiforme. Cancer Diagn Progn. 2021; 1(2): 77-82.
11.
Sooda K, Allison SJ, Javid FA. Investigation of the cytotoxicity induced by cannabinoids on human ovarian carcinoma cells. Pharmacol Res Perspect. 2023; 11(6): e01152.
12.
Tilsed CM, Fisher SA, Nowak AK, Lake RA, Lesterhuis WJ. Cancer chemotherapy: insights into cellular and tumor microenvironmental mechanisms of action. Front Oncol. 2022; 12: 960317.
13.
Shokrzadeh Madieh N, Tanna S, Alqurayn NA, Vaidea- nu A, Schatzlein A, Brucoli F. Aminobenzofuran-containing analogues of proximicins exhibit higher antiproliferative activity against human UG-87 glioblastoma cells compared to temozolomide. RSC Adv. 2023; 13(12): 8420-8426.
14.
Carmichael J, Mitchell JB, DeGraff WG, Gamson J, Gaz- dar AF, Johnson BE, Glatstein E, Minna JD. Chemosensitivity testing of human lung cancer cell lines using the MTT assay. Br J Cancer. 1988; 57(6): 540-547.
15.
Alexandru O, Georgescu AM, Ene L, Purcaru SO, Serban F, Popescu A, Brindusa C, Tataranu LG, Ciubotaru V, Dri- cu A. The effect of curcumin on low-passage glioblastoma cells in vitro. J Cancer Res Ther. 2016; 12(2): 1025-1032.
16.
Kulpa J, Harrison A, Rudolph L, Eglit GML, Turcotte C, Bonn-Miller MO, Peters EN. Oral cannabidiol treatment in two postmenopausal women with osteopenia: a case series. Cannabis Cannabinoid Res. 2023; 8(S1): S83-S89.
17.
Wang T, Zakharov A, Gomez B, Lyubimov A, Trottier NL, Schwark WS, Wakshlag JJ. Serum cannabinoid 24 h and 1 week steady state pharmacokinetic assessment in cats using a CBD/CBDA rich hemp paste. Front Vet Sci. 2022; 9: 895368.
18.
McGrath S, Bartner LR, Rao S, Packer RA, Gustafson DL. Randomized blinded controlled clinical trial to assess the effect of oral cannabidiol administration in addition to conventional antiepileptic treatment on seizure frequency in dogs with intractable idiopathic epilepsy. J Am Vet Med Assoc. 2019; 254(11): 1301-1308.
19.
Greco R, Francavilla M, Demartini C, Zanaboni AM, Sodergren MH, Facchetti S, et al. Characterization of the biochemical and behavioral effects of cannabidiol: implications for migraine. J Headache Pain. 2023; 24: 48.
20.
Williamson EM, Evans FJ. Cannabinoids in clinical practice. Drugs. 2000; 60(6): 1303-1314.
21.
Al-Khazaleh AK, Zhou X, Bhuyan DJ, Münch GW, Al-Dalabeeh EA, Jaye K, Chang D. The neurotherapeutic arsenal in cannabis sativa: insights into anti-neuroinflammatory and neuroprotective activity and potential entourage effects. Molecules. 2024; 29(2): 410.
| eISSN: | 2300-6722 |
| ISSN: | 1899-1874 |
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